设为首页  |  加入收藏  |   产品世界

搜索

联系我们   CONTACT US

可信组件

联系人:高女士

联系电话: 18602670353   

Email:gaopei@okinst.com

详细地址:天津 天津市红桥区光荣道157号宝能创业中心A座13层 

版权所有:天津东方科捷科技有限公司             网站建设:中企动力  天津             津ICP备13002962号

快速链接   QUICK LINKS

手机官网

>
>
>
FLIM升级模块--配合Nikon 共焦显微镜

FLIM升级模块--配合Nikon 共焦显微镜

浏览量
产品名称

FLIM升级模块--配合Nikon 共焦显微镜

没有此类产品
产品描述

Nikon, FCS and FLIM

 

 

The Nikon A1 and C2 confocal microscopes series can be easily upgraded to Fluorescence Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS) capability using photon counting detection. Data are acquired using one of two modalities:

  • the FastFLIM (digital frequency domain); or
  • the TCSPC (time-domain).

FastFLIM (proprietary technology) is the new digital frequency domain (DFD) approach to FLIM measurements. Its advantages, when compared to the TCSPC are twofold:

a) the short time required for data acquisition; and
b) the higher sensitivity of the technique (due to the 100% duty-cycle).

Alternatively, FLIM data can be acquired using the TCSPC method, or both methodologies can be implemented on the same instrument.

The Nikon, FCS and FLIM upgrade package includes the following items:

FastFLIM Unit or TCSPC Unit It accepts the output (via BNC) from up to four PMTs of the confocal unit. The synchronization signal from the Nikon confocal head is connected to the unit.
Detectors 2-detectors coupled to the descanned port confocal head of the LSM microscope; or to the non-descanned port (for multiphoton instruments)
Detectors fast PMTs
Laser Launcher Available for 3-, 4- and 6-lasers. The lasers beams are superimposed and the output of the laser launcher is connected to the microscope by using a fiber optic.
Computer Running VistaVision by ISS A separate computer, with a 27" flat monitor

 

 

 

 

 

 

 

 

Acquisition and Analysis Software
Fluorescence Fluctuations Spectroscopy (FFS) Measurements
  • Fluorescence Correlation Spectroscopy (auto- and cross-correlation)
  • Photon Counting Histogram (PCH)
  • FFS measurements at target XYZ locations in an image
  • FLCS, Fluorescence Lifetime Correlation Spectroscopy
  • Number & Brightness (N&B)
Imaging Module Measurements
  • Single-point (intensity, polarization, lifetime)
  • Single plan and z-stack (polarization images, Ratiometric, FLIM)
FLIM images (digital frequency-domain) (single plane and z-stack)
  • Acquired in digital frequency-domain (DFD). The routine acquires simultaneously a FLIM image and a steady-state image.
FLIM images time-domain (single plane and z-stack)
  • Acquired in time-correlated single photon counting (TCSPC)
Single Molecule Module
  • Burst Analysis
  • FRET and Correlation Methods
  • PIE-FRET Methods
Light Sources
  • Laser diodes: 370 nm - 1000 nm
  • Single-photon pulsed lasers
  • Multi-photon lasers
Laser Launcher
  • Models for 3-, 4-, 6-laser. Light is delivered to the microscope through a single-mode fiber optic.
Input Channels
  • Two
Detectors
  • GaAs PMT (Model H7422P)
  • Hybrid PMTs (Model R10467U)
CLK
  • Pixel, Line, Frame
FLIM Image Data Acquisition Minimum Dwell Time
  • 6 µs/pixel
Unit Control
  • USB
Computer
  • 3 GHz, 16GB RAM, 27" monitor 2556 x 1440 resolution
  • Windows 10, 64-bit

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 1. Schematics of the upgrade package for the Nikon Frequency-Domain Confocal Microscope.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Figure 2. Schematics of the upgrade package for the Nikon Time-Domain Confocal Microscope.

Below is a list of selected publications featuring the Nikon, FCS and FLIM upgrade from ISS.

 

Photoluminescence Enhancement and High Accuracy Patterning of Lead Halide Perovskite Single Crystals by MeV Ion Beam Irradiation
Palei, M., Motapothulab, M, Ray A., Abdelhadya, A.L., Lanzanod, L., Prato, M., Panda, J.K., Scarpellini, A., Pellegrinif, V., Primetzhofer, D., Petralanda, U., Manna, L., Dang, Z.
J. Mater. Chem. C, 2020,8, 9923-9930
Chromatin nanoscale compaction in live cells visualized by acceptor-to-donor ratio corrected Förster resonance energy transfer between DNA dyes.
Pelicci, S., Diaspro, A., Lanzanò, L.
J Biophotonics. 2019 Jul 31:e201900164. doi: 10.1002/jbio.201900164. [Epub ahead of print]

 

未找到相应参数组,请于后台属性模板中添加
暂未实现,敬请期待
暂未实现,敬请期待
上一篇
下一篇

PRODUCT CENTER

产品中心