FLIM升级模块--配合Olympus 共焦显微镜

所属分类

激光共聚焦荧光成像系统 & FLIM

产品描述

Olympus, FCS and FLIM Upgrade

The FV1000, FV1200, FV3000 and FVMPE-RS confocal microscopes systems can be easily upgraded to Fluorescence Lifetime Imaging (FLIM) and Fluorescence Correlation Spectroscopy (FCS) capability using photon counting detection. Data are acquired using one of two modalities:

the FastFLIM (digital frequency domain); or
the TCSPC (time-domain).

FastFLIM (proprietary technology) is the new digital frequency domain (DFD) approach to FLIM measurements. Its advantages, when compared to the TCSPC are twofold:

a) the short time required for data acquisition; and
b) the higher sensitivity of the technique (due to the 100% duty-cycle).

The Olympus FCS and FLIM Upgrade Kit includes the following items:

FastFLIM Unit or TCSPC Unit	It accepts the output (via BNC) from up to four PMTs of the confocal unit. The synchronization signal from the Olympus confocal head is connected to the unit.
Detectors	Options of: Internal detectors 2-detector unit coupled to the fiber optic port of the microscope 2-detectors coupled to the confocal head of the microscope
Detectors	fast PMTs or SPADs.
Laser Launcher	Available for 3-, 4- and 6-lasers. The lasers beams are superimposed and the output of the laser launcher is connected to the microscope by using a fiber optic.
Computer Running VistaVision by ISS	A separate computer, with a 27" flat monitor

Specifications

Acquisition and Analysis Software	VistaVision by ISS
Fluorescence Fluctuations Spectroscopy (FFS) Module Measurements	Fluorescence Correlation Spectroscopy (auto- and cross-correlation) Photon Counting Histogram (PCH) FFS measurements at target XYZ locations in an image FLCS, Fluorescence Lifetime Correlation Spectroscopy Scanning FCS Number & Brightness (N&B)
Imaging Module Measurements	Single-point (intensity, polarization, lifetime) Single plan and z-stack (polarization images, ratiometric, FLIM)
Superresolution Module	Particle tracking Nanoimaging
FLIM images (digital frequency-domain) (single plane and z-stack)	Acquired in digital frequency-domain (DFD). The routine acquires simultaneously a FLIM image and a steady-state image.
FLIM images time-domain (single plane and z-stack)	Acquired in time-correlated single photon counting (TCSPC)
Single Molecule Module	Burst Analysis FRET and Correlation Methods PIE-FRET Methods
Light Sources	Laser diodes: 370 - 1000 nm Ti:Sapphire lasers
Laser Launcher	Models for 3-, 4-, 6-lasers. Light is delivered to the microscope through a single-mode fiber optic.
Input Channels	Two
Detectors	Internal (original) PMTs External GaAs PMT (Model H7422P) Hybrid PMT (Model R10467U)
CLK	Pixel, Line, Frame
FLIM Image Data Acquisition Minimum Dwell Time	4 µs/pixel
Unit Control	USB
Computer	3 GHz, 8GB RAM, 27" monitor 2556x1440 resolution Windows 10, 64-bit

The upgrade can be carried out following one of two modalities:

a) By adding two external PMTs (2-detector unit); the fluorescence signal is collected through the fiber port of the microscope and diverted to the 2-detector unit (Figure 1)
b) Utilize the internal PMTs of the FV1000 (Figure 2)

Figure 1. Schematics of the upgrade package for the Olympus confocal microscope. The part to the right includes the instrument components (PC, control electronics, scanner and laser launcher). The left part of the schematics includes the components provided by ISS with the upgrade package.

Figure 2. Schematics of the upgrade package for the Olympus FV1000. The part to the right includes the instrument components (PC, control electronics, scanner and laser launcher). The signal is collected from the internal detectors of the microscope and diverted to FastFLIM unit (left part) of the schematics which includes the components provided by ISS with the upgrade package.

用户发布论文：

	Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures. Hato, T., Winfree, S., Day, R., Sandoval, R.M., Molitoris, B.A., Yoder, M.C., Wiggins, R.C., Zheng, Y., Dunn, K.W., Dagher, P.C. J Am Soc Nephrol. 2017 Mar 1. pii: ASN.2016101153. doi: 10.1681/ASN.2016101153. [Epub ahead of print]
	Imaging Sensitivity of Quiescent Cancer Cells to Metabolic Perturbations in Bone Marrow Spheroids. Cavnar, S.P., Xiao, A., Gibbons, A.E., Rickelmann, A.D., Neely, T., Luker, K.E., Takayama, S., Luker, G.D. Tomography. 2016 Jun;2(2):146-157.
	Fluorescence Lifetime Imaging of Apoptosis. Xiao, A., Gibbons, A.E., Luker, K.E., Luker, G.D. Tomography. 2015 Dec;1(2):115-124.

FLIM升级模块--配合Nikon 共焦显微镜

FLIM升级模块--配合Leica TCS SP8 共焦显微镜

PRODUCT CENTER

产品中心