ISS Q2 模块化荧光寿命成像系统FLIM/FCS

基于激光共焦扫描显微系统--荧光强度扫描成像、荧光寿命扫描成像FLIM/PLIM、单分子扫描成像FCS

　　新一代快速荧光寿命成像FastFLIM系统，提供单分子级的灵敏度，检测波长标准配置范围：350-1050nm；寿命范围100ps-100ms；

  用于化学、纳米、能源、生物等学科方向，单分子动态、活细胞、微区成像及形貌、能级结构和能量传递特征的机理研究，测试速度高达5fps（512×512），1×1 到 4096×4096成像，采用可视化Phasor plots荧光寿命直读半圆规，批量荧光寿命成像数据处理进入直读时代；振镜扫描适于所有时间尺度的寿命成像。光谱测量，活细胞工作站、液氮变温测试可选用；

 

The compact instrument for quantitative cell biology with single- molecule detection.

Q2 is a laser scanning nanoscope (LSN) that incorporates several measurement modalities for experimental quantitative biology and material sciences applications requiring the single molecule detection sensitivity.

Key Features of Q2 Laser Scanning Nanoscope:

• Single- and multi-photon excitation on three separate input ports
• Two channel acquisition
• One, computer-controlled-aperture, pinhole

• Fast scanning mirrors
• Powered by VistaVision, a user-friendly software package for the acquisition of confocal images, FLIM/ FRET, RICS, and FFS (FCS, PCH, scanning FCS, N&B)

 

 

 

 

Measurements with Q2:

• 1p or 2p confocal images
• FLIM Fluorescence Lifetime Imaging (FastFLIM or TCSPC)
• PLIM, Phosphorescence Lifetime Imaging
• Polarization images
• Fluorescence Fluctuations Spectroscopy (FCS, FCCS, PCS, FLCS)
• RICS, N&B, Scanning FCS
• Burst Analysis
• FRET efficiency determination
• 3D particle tracking trajectories
• Nanoimaging reconstruction with 20 nm resolution

 

 

 

 

 

 

 

 

Specifications for Q2

Microscope and Coupling	Olympus, Nikon, Zeiss and Leica Inverted and upright Left side port and back port
1p Excitation1	ISS laser launcher (models for 3-, 4-, and 6-lasers), wavelengths available from 375 nm to 1000 nm; pulse interleave excitation (PIE) Supercontinuum lasers, wavelength from 400 to 950 nm
2p Excitation1	Ultra fast femtosecond pulse Ti:Sapphire lasers Ultra fast femtosecond pulse fiber lasers
Data Acquisition Unit	FastFLIM (Digital Frequency Domain FLIM) Supports all above listed applications except particle tracking and nanoimaging TCSPC card (Time Domain FLIM)
Detectors	GaAs PMT (Hamamatsu H7421 and H7422P models) Hybrid PMTs (Hamamatsu R10467U models) SPADs
Positioning Controls2	ISS 3-axis control unit ISS XY galvo scanning mirrors control unit ISS Z-piezo control unit Microscope built-in focusing control module Automatic XY stages (ASI, Prior)
Software	VistaVision
Computer & Monitor	3GHz Processor, 16GB RAM, 64-bit VistaVision 27", 2556 x 1440 resolution monitor Windows 10 64-bit Professional
Power Requirements	Universal power input: 110-240 V, 50/60 Hz, 400 VAC

 

1. Q2 was fully evaluated and validated for using ISS laser launcher, Fianium SC-400 laser and Toptica FemtoFiber Pro 2p laser.
2. VistaVision provides utilities for measurements in spectroscopy mode (at a single point), raster or orbit scan mode (2D XY), optical sectioning mode (3D), time-lapse mode, stage scan mode for multi wells, or a combination of them.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Measurements for Q2

 

 

代表性论文：

 

	Multiplexed patterning of hybrid lipid membrane and protein arrays for cell signaling study Ti, Y.-T., Cheng, H.-C., Lia, Y., Tu, H.-L. Lab Chip, 2021, 21, 2711-2720
	Low-Dimensional Organic Metal Halide Hybrids with Excitation-Dependent Optical Waveguides from Visible to Near-Infrared Emission Wu, S., Zhou, B., Yan, D. ACS Appl. Mater. Interfaces, 2021, 13, 22, 26451–26460
	Near-Unity Cyan-Green Emitting Lead-Free All-Inorganic Cesium Copper Chloride Phosphors for Full-Spectrum White Light-Emitting Diodes Bai, W., Shi, S., Lin, T., Zhou, T., Xuan, T., Xie, R. Adv. Photonics Res., 2021, 2: 2000158.
	Genetic encoding of a highly photostable, long lifetime fluorescent amino acid for imaging in mammalian cells Jones, C.M., Robkis, D.M., Blizzard, R.J., Munari, M., Venkatesh, Y., Mihaila, T.S., Eddins, A.J., Mehl, R.A., Zagotta, W.N., Gordon, S.E., Petersson, E.J. Chem. Sci. 2021. http://doi.org/10.1039/d1sc01914g
	Nanoscopic Insights of Amphiphilic Peptide against the Oligomer Assembly Process to Treat Huntington's Disease He, R.‐Y., Lai, X.‐M., Sun, C.‐S., Kung, T.‐S., Hong, J.‐Y., Jheng, Y.‐S., Liao, W.‐N., Chen, J.‐K., Liao, Y.‐F., Tu, P.‐H., Huang, J. J.‐T. Adv. Sci. 2019, 1901165. https://doi.org/10.1002/advs.201901165
	Concurrent Exposure of Neutralizing and Non-neutralizing Epitopes on a Single HIV-1 Envelope Structure Ray, K., Mengistu, M., Orlandi, C., Pazgier, M., Lewis, G.K., DeVico, A.L. Front. Immunol., 10:1512, 2019 July.
	Band-Aligned Polymeric Hole Transport Materials for Extremely Low Energy Loss α-CsPbI3 Perovskite Nanocrystal Solar Cells Yuan, J., Ling, X., Yang, D., Li, F., Zhou, S., Shi, J., Qian, Y., Hu, J., Sun, Y., Yang, Y., Gao, X., Duhm, S., Zhang, Q., Ma, W. Joule, ISSN: 2542-4351, 2018 Sep 10.
	Composition‐Graded Cesium Lead Halide Perovskite Nanowires with Tunable Dual‐Color Lasing Performance Huang, L., Gao, Q., Sun, L.-D., Dong, H., Shi, S., Cai, T., Liao, Q., Yan, C.-H. Advanced Materials. 2018 May 21.
	Targeting the Late Stage of HIV-1 Entry for Antibody-Dependent Cellular Cytotoxicity: Structural Basis for Env Epitopes in the C11 Region. Tolbert, W.D., Gohain, N., Alsahafi, N., Van, V., Orlandi, C., Ding, S., Martin, L., Finzi, A., Lewis, G.K., Ray, K., Pazgier, M. Structure. 2017 Nov 7;25(11):1719-1731.e4. doi: 10.1016/j.str.2017.09.009. Epub 2017 Oct 19.
	Molecular basis for epitope recognition by non-neutralizing anti-gp41 antibody F240. Gohain, N., Tolbert, W.D., Orlandi, C., Richard, J., Ding, S., Chen, X., Bonsor, D.A., Sundberg, E.J., Lu, W., Ray, K., Finzi, A., Lewis, G.K., Pazgier, M. Sci Rep. 2016 Nov 9;6:36685. doi: 10.1038/srep36685.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

主要功能描述：

激光共焦荧光强度成像LCM；

荧光寿命成像FLIM，磷光寿命成像PLIM；

上转换荧光（寿命）成像，稀土发光（寿命）成像，延迟荧光（寿命）成像；

荧光波动成像FFS（FCS，FCCS, PCH，N&B, RICS， FLCS），FLIM-FRET成像；

单量子点发光（寿命）成像，单分子及单分子荧光共振转移成像，包括交替激发PIE成像；

稳态及瞬态偏振成像；

微区荧光光谱采集及光谱成像；

反聚束测试；

　　数字频域技术DFD-FLIM （FastFLIM）和时域技术TD-FLIM （TCSPC）成像技术；

　　实时直读式获得荧光寿命数值及变化趋势，FRET-FLIM 效率分布；

　　选择300-1600nm波长范围检测器，2-4通道检测器，用于成像，FLIM-FRET；

　　可以升级无波长干扰AFM，实现同区域形貌和FLIM同步测试；

   紫外-可见-红外激发波长，单波长或超连续激光器；266nm-1300nm激光器可选；PIE功能可选；白光激光器可选用；

　　单光子或双光子的激光器；